CRISPR4P: CRISPR Pombe PCR Primer Program (or CRISPR ‘for’ Pombe)

• CRISPR4P is a web-tool to design sgRNAs and primers required for deletions using CRISPR/Cas9.

AnGeLi: Analysis of Gene Lists

• AnGeLi is a tool for gene set enrichment analysis.

Spotsizer: Software for quantitative analysis of microbial growth

• Spotsizer is a tool for high-throughput quantification of colony sizes.

Gene Expression Viewer

• GE Viewer is a visualization tool to look up gene expression profiles in different conditions.

pombeTV: pombe Transcriptome Viewer

• pombeTV is a tool for displaying tiling chip and next-generation sequencing data for genomic regions.

PInt: Pombe Interactome

• PInt is a tool that allows you to retrieve predictions of fission yeast protein interactions, based on more than 100 features of the proteins.

YOGY: eukarYotic OrtholoGY

• YOGY is an integrated resource to search for orthologous proteins in 11 organisms.

PPPP: Pombe PCR Primer Programs

Web-interface scripts to design primers for PCR-based gene targeting in S. pombe

• Gene Deletion
• C-terminal tagging
• Get primers for controlling genes by nmt promoter and/or N-terminal tagging

Microarray Data

• Meta-analysis

• Meiotic differentiation

• Stress Responses

• Cell Cycle

• Other Projects

Protocols

• Protocols we use for microarray experiments.

Presentations

• Presentations available as PDF files.

Other scripts

These scripts are for primer design and initial microarray data processing and are available as .zip files:

• Initial microarray data processing (data filtering, local normalisation and quality control)
• Intragenic Microarray Primer design
• Intergenic Microarray Primer design

Microarray Primer Database

• Primer Database to retrieve the sequences and accompanying information for primers and PCR products used for our in-house arrays.